ELISA試劑盒發表的文獻集錦
更新時間:2022-11-10 點擊次數:453次ELISA試劑盒發表的文獻集錦
信帆生物供應各類elisa試劑盒,提供免費代測,上門取樣,送貨上門服務!本公司產品質量穩定,庫存充足,現貨供應。
操作步驟
從室溫平衡60min后的鋁箔袋中取出所需板條,剩余板條用自封袋密封放回4℃。
設置標準品孔和樣本孔,標準品孔各加不同濃度的標準品50μL;
待測樣本孔先加待測樣本10μL,再加樣本稀釋液40μL;
隨后標準品孔和樣本孔中每孔加入辣根過氧化物酶(HRP)標記的檢測抗體100μL,用封板膜封住反應孔,37℃水浴鍋恒溫箱溫育60min。
棄去液體,吸水紙上拍干,每孔加滿洗滌液,靜置1min,甩去洗滌液,吸水紙上拍干,如此重復洗板5次(也可用洗板機洗板)。
每孔加入底物A、B各50μL,37℃避光孵育15min。
每孔加入終止液50μL,15min內,在450nm波長處測定各孔的OD值。
操作注意事項
試劑盒保存在2-8℃,使用前室溫平衡60分鐘。從冰箱取出的濃縮洗滌液會有結晶,這屬于正常現象,水浴加熱使結晶溶解后再使用。樣本在使用前也要在室溫平衡60分鐘。
實驗中不用的板條應立即放回自封袋中,密封(低溫干燥)保存。
預處理后的樣本無需稀釋,直接取10μL加樣即可。
嚴格按照說明書中標明的時間、加液量及順序進行溫育操作。
所有液體組分使用前充分搖勻。
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ELISA試劑盒發表的文獻集錦ELISA試劑盒發表的文獻集錦